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@#AIM:To investigate the relationship between dry eyes incidence and poor vision in primary and middle school students in the urban-rural fringe of Guangzhou. <p>METHODS: We used stratified cluster random sampling, extracted the urban-rural fringe elementary school and middle school, gave out self-made questionnaire survey to 3572 students, at the same time, underwent ophthalmology routine inspection and tear secretion test, rupture time measurement and corneal and conjunctival fluorescein staining. <p>RESULTS: The overall number of poor vision was 764 people(1447 eyes), the overall poor vision was 20.25% counted by eye amount, the rate of poor vision in primary school was 14.08%, in junior high school was 29.51%, in junior middle school was significantly higher than that in primary school(<i>P</i><0.05). The number of subjects with dry eyes was 424 cases(848 eyes), the overall incidence of dry eyes was 11.87%, among them the occurring rate of dry eyes in primary school was 5.14%, in junior middle school was 21.96%, which was significantly higher than that in primary school(<i>P</i><0.05). Ordered multivariate Logistic regression analysis showed that junior high school, doing homework for 1-3h or >3h every day, eyes away from the book <30cm, lying down when reading books, watching TV or playing computer phone for 1-3h and >3h every day, dry eyes, and the nutritional status(fat or thin)were the risk factors of poor vision in primary and middle school students the(<i>OR</i>=3.862, 1.164, 1.210, 1.212, 1.315, 1.319, 3.040, 1.110, 1.057 and 1.141). <p>CONCLUSION: The risk factors of primary and middle school students vision is multifarious, dry eyes is a risk factor for poor vision of primary and middle school students in the urban-rural fringe of Guangzhou.
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<p><b>OBJECTIVE</b>To study the effect of LY294002 on the adriamycin- induced epithelial-mesenchymal transition in human breast carcinoma cells.</p><p><b>METHODS</b>Human breast carcinoma cells MCF-7 was cultured in vitro and then exposed to adriamycin with or without LY294002. The protein expression levels of Akt, phosphorylated-Akt (p-Akt), Snail, and E-cadherin was detected by Western blot analysis. The mRNA expressions of Snail and E-cadherin were determined by RT-PCR.</p><p><b>RESULTS</b>Adriamycin significantly increased the protein expression of Snail and depressed the protein expression of E-cadherin (P<0.05). The pre-treatment with LY294002 significantly reversed the changes of activities and levels of the above proteins (P<0.05).</p><p><b>CONCLUSION</b>LY294002 could reverse the adriamycin-induced epithelial-mesenchymal transition in human breast carcinoma cells by regulating the expressions of Snail and E-cadherin through suppressing PI3K/Akt signaling pathway.</p>
Subject(s)
Humans , Breast Neoplasms , Metabolism , Pathology , Cadherins , Metabolism , Chromones , Pharmacology , Doxorubicin , Pharmacology , Epithelial-Mesenchymal Transition , MCF-7 Cells , Morpholines , Pharmacology , Phosphatidylinositol 3-Kinases , Proto-Oncogene Proteins c-akt , Metabolism , Signal Transduction , Snail Family Transcription Factors , Transcription Factors , MetabolismABSTRACT
0.05].After 12 week treatment,the YGTSS total score in study group was significantly lower [(27.88?14.24) vs(36.53?17.45) scores] and the reduction rate of YGTSS score was significantly higher [(43.12?18.42)% vs(33.49?14.38)%] compared with those in control group(Pa
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Objective To investigate the changes of umbilical adiponectin(APN) levels in newborn whose mothers with gestational impaired glucose tolerance (GIGT).Methods Eighteen neonates of GIGT pregnancies and 50 controls of normal glucose tolerance (NGT) pregnancies were studied.APN concentration was qualified in the umbilical vein serum with radioimmunoassay methods.Capillary blood glucose was measured at the first hour after delivery.Results Umbilical APN level in the GIGT group was significantly higher than that in NGT group [(61.06?16.85)mg/L vs(48.04?15.47)mg/L,t=2.38 P0.05).Conclusions Umbilical serum APN levels are elevated in newborns of GIGT mo-thers.This may be due to the dysregulation of serum APN levels associate with GIGT pregnancy.More attention shall be paid to the metabolic changes of GIGT mothers and their offspring.